Intsholongwane yeDNA / iRNA yokuThintela (ikholamu)

Ikhithi yokuCocwa kweDNA isetyenziselwa ukukhutshwa okulula, okukhawulezayo kunye nokuthembekileyo kwe-DNA ejikeleza simahla (i-CFC-DNA) ukusuka kwi-1 ml ukuya kwi-4 ml yeplasma / serum. Indlela yokuhlanjululwa isekwe kwikholamu ye-chrtatografi kwaye isebenzisa i-resin ye-Sigma yokuthengisa ukwahlula i-matrix. Ikhithi inokusetyenziselwa ukwahlula i-CFC-DNA yobungakanani obahlukeneyo kwiisampulu zeplasma / ze-serum ezintsha okanye ezinqabileyo. Ukongeza, ivolumu yokuqaqamba inokuhlengahlengiswa ngokulula ukusuka kwi-25 μl ukuya kwi-50 μl. Iplasma / i-serum CFC-DNA ecociweyo iyakudityaniswa kwi-eluent ukuze ihambelane nazo naziphi na izicelo ezisezantsi, kubandakanya i-PCR, i-PCR yokwenyani yexesha lokwenyani, i-PCR ye-methylation ethe-ethe kunye nohlalutyo lwe-blot eseMazantsi, imicroarray kunye ne-NGS.

Ukujikeleza kwe-DNA ngobukhulu obahlukeneyo kunokuhlukaniswa kwiisampulu zeplasma kunye neserum
I-DNA yentsholongwane kunye nebacteria inokuchongwa
Zilungele zonke iintlobo zeplasma kunye neserum ukuqala kwesampulu (1 mL ~ 4 mL)
Umthamo wokulungiswa unokuhlengahlengiswa ngokuguqukayo kuluhlu lwe-50 μL ~ 100 μL ukugxila ekujikelezeni kwe-DNA
Ukuhanjiswa simahla kwe-DNA ngaphandle kwesithintelo kunokwahlulwa
Umgangatho ophezulu weDNA unokucocwa ngemizuzu engama-40-45
Iyahambelana neeTyhubhu zeDNA eziSimahla zeDNA BCT

Ukusetyenziswa

I-PCR
qPCR
Ushicilelo olusemazantsi
I-PCR ebuthathaka yeMethylation
Uluhlu lweCpG
Ukuthintela ukwetyisa i-enzyme
Ukufumanisa intsholongwane
Ukufunyanwa kwebhakteria
encinci
NGS